TY - JOUR AU - Unver, Nese AU - Yilmaz, Guldal AU - Canpinar, Hande AU - Guc, Dicle AU - Esendagli, Gunes PY - 2018/03/31 Y2 - 2024/03/29 TI - Regulatory Role of Chemerin in Lung Tumorigenesis and Cancer-associated Inflammation: Anti-inflammatory effect of chemerin in cancer JF - Acta Medica JA - Acta Medica VL - 49 IS - 1 SE - Original Article DO - UR - https://actamedica.org/index.php/actamedica/article/view/278 SP - AB - <p style="font-weight: 400;">Objective:&nbsp;Interaction between the chemokine-like receptor 1 (CMKLR1) and its cognate peptide ligand, chemerin, can downregulate the inflammatory responses. This study investigates the regulatory role chemerin in lung tumorigenesis and cancer-associated inflammation.</p><p style="font-weight: 400;">Materials and methods:&nbsp;Animal experiments, cell culture, gene cloning and transfection, chemotaxis assay, Lewis lung carcinoma (LLC) tumorigenesis assays, histopathological evaluation, mixed leukocyte reactions, flow cytometry, proliferation and clonogenicity assays, reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry (IHC) techniques were used.</p><p style="font-weight: 400;">Results:&nbsp;CMKLR1 was identified in mouse LLC tumors. The LLC cells were modified to secrete prochemerin at a level that cannot induce chemotactic activity. Inflammatory cytokine expression, TNF-α, IL-12 p40, but not IL-1β, was diminished in leukocytes when exposed to conditioned media obtained from the chemerin-expressing LLC cells, and in the tumors established with those cells. On the other hand, leukocyte proliferation and IFN-γ expression were not drastically impeded. Correspondingly, tumor forming capacity of the chemerin-expressing LLCs was decreased.</p><p style="font-weight: 400;">Conclusion<strong>:</strong>&nbsp;Chemerin may possess a potential to modulate the inflammatory responses in the tumor microenvironment.</p> ER -